Dr. Konstantina T Tsoumani

About Dr. Konstantina T Tsoumani

Dr. Konstantina T is a Archives of insect biochemistry and physiologyREFERENCES researcher from Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.. His recent research is on Developing CRISPR-based sex-ratio distorters for the genetic control of fruit fly pests: A how to manual. which was published on Arch Insect Biochem Physiol on 2019/12/17.

The DOI of the article Developing CRISPR-based sex-ratio distorters for the genetic control of fruit fly pests: A how to manual. is 10.1002/arch.21652

The article has been published in the following journal:
United States Arch Insect Biochem Physiol 8501752 0739-4462

Co-Authors:


List of co-authors for Developing CRISPR-based sex-ratio distorters for the genetic control of fruit fly pests: A how to manual. :
Angela Meccariello
Kostas D Mathiopoulos
Philippos Aris Papathanos

What are Dr. Konstantina T Tsoumani Specialties :


CRISPR/Cas9 Redkmer X-chromosome X-shredding system spermatogenesis

List of Publications:

  1. De Novo Assembly of the Olive Fruit Fly (Bactrocera Oleae) Genome With Linked-Reads and Long-Read Technologies Minimizes Gaps and Provides Exceptional Y Chromosome Assembly
      The high-quality genome generated represents a critical tool in olive fruit fly research. We provide an extensive RNA-seq data set, and genome annotation, critical towards gaining an insight into the biology of the olive fruit fly. In addition, elucidation of Y-chromosome sequences will advance our …
  2. Targeted Somatic Mutagenesis Through CRISPR/Cas9 Ribonucleoprotein Complexes in the Olive Fruit Fly, Bactrocera Oleae
      The olive fruit fly, Bactrocera oleae (Diptera: Tephritidae), is the most destructive insect pest of olive cultivation, causing significant economic and production losses. Here, we present the establishment of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 methodology fo …
  3. Developing CRISPR-based Sex-Ratio Distorters for the Genetic Control of Fruit Fly Pests: A How to Manual
      Agricultural pest control using genetic-based methods provides a species-specific and environmentally harmless way for population suppression of fruit flies. One way to improve the efficiency of such methods is through self-limiting, female-eliminating approaches that can alter an insect populations …
  4. Maleness-on-the-Y ( MoY) Orchestrates Male Sex Determination in Major Agricultural Fruit Fly Pests
      In insects, rapidly evolving primary sex-determining signals are transduced by a conserved regulatory module controlling sexual differentiation. In the agricultural pest <i>Ceratitis capitata</i> (Mediterranean fruit fly, or Medfly), we identified a Y-linked gene, <i>Maleness-on-the-Y</i> (<i>MoY</i> …
  5. Housekeeping in Tephritid Insects: The Best Gene Choice for Expression Analyses in the Medfly and the Olive Fly
      Real-time quantitative-PCR has been a priceless tool for gene expression analyses. The reaction, however, needs proper normalization with the use of housekeeping genes (HKGs), whose expression remains stable throughout the experimental conditions. Often, the combination of several genes is required …
  6. Achilles, a New Family of Transcriptionally Active Retrotransposons From the Olive Fruit Fly, With Y Chromosome Preferential Distribution
      Sex chromosomes have many unusual features relative to autosomes. The in depth exploration of their structure will improve our understanding of their origin and divergence (degeneration) as well as the evolution of genetic sex determination pathways which, most often are attributed to them. In Tephr …
  7. The Molecular Biology of the Olive Fly Comes of Age
      Several years of molecular studies on the olive fly can now be combined with new information from whole transcriptome analyses and lead to a deep understanding of the biology of this notorious insect pest. This is a prerequisite for the development of novel embryonic lethality female sexing strains …
  8. Olive Fly Transcriptomics Analysis Implicates Energy Metabolism Genes in Spinosad Resistance
      The observed nucleotide differences in the nAChR-α6 subunit between the sensitive and spinosad resistant olive fly strains did not advocate for the involvement of receptor mutations in spinosad resistance. Instead, the transcriptome comparison between the two strains indicated that several immune sy …
  9. Molecular Characterization and Chromosomal Distribution of a Species-Specific Transcribed Centromeric Satellite Repeat From the Olive Fruit Fly, Bactrocera Oleae
      Satellite repetitive sequences that accumulate in the heterochromatin consist a large fraction of a genome and due to their properties are suggested to be implicated in centromere function. Current knowledge of heterochromatic regions of Bactrocera oleae genome, the major pest of the olive tree, is …
  10. Spinosad Resistance Development in Wild Olive Fruit Fly Bactrocera Oleae (Diptera: Tephritidae) Populations in California
      The observed resistance increase was associated with spinosad applications in the respective areas. These values are relatively low and do not yet pose a serious control problem in the field. However, the observed variation documents that spinosad tolerance has increased in areas where the insectici …

Keywords :


CRISPR/Cas9 Redkmer X-chromosome X-shredding system spermatogenesis